Anti-larvicidal Potential of Citrus aurantifolia bark extract for Sustainable control of Mechanical Vectors of human parasitic diseases
Ndejje University Journal Of Interdisciplinary Studies,
Volume 2, Issue 2
eISSN: 3079-7683
DOI: https://doi.org/10.64080/ndujis.2026.2.2.Oct005
1.Moses Oluwaseun Abiodun, Department of Public Health, Faculty of Basic Medical Sciences, Osun State University Osogbo, Nigeria. Email: moses.abiodun@uniosun.edu.ng or moskad92@gmail.com ORCID https: //orcid.org/0009-0009-3194-1384
2.Maryjude Chiamaka Igbodika Department of Public Health, Faculty of Basic Medical Sciences, Osun State University Osogbo, Nigeria. Email: chiamakamaryjude@coou.ed.ng ORCID https: //orcid.org/0005-0009-5674-4631
3.Taofik Olatunji Bankole. Department of Public Health, Faculty of Basic Medical Sciences, Osun State University Osogbo, Nigeria. Email: olatunji.bankole@uniosun.edu.ng ORCID: https:// orcid.org/0000-0002-9366-0416
4.Calistus Adewale Akinleye Department of Public Health, Faculty of Basic Medical Sciences, Osun State University Osogbo, Nigeria. Email: calistus.akinleye@uniosun.edu.ng ORCID: https:// orcid.or// 0000-0003-37778-3638
5.Sanuel Adeyinka Adedokun Department of Public Health, Faculty of Basic Medical Sciences, Osun State University Osogbo, Nigeria. Email: Samuel.adekunle@uniosun.ed.ng ORCID: https:// orcid.org/ 0000-0001-8148-7305
6.Emeka.Christopher Anaebnam Department of Public Health, Faculty of Basic Medical Sciences, Osun State University Osogbo, Nigeria. Email: Benedicthospital2007@yahoo.com ORCID: https:// orcid.org/0002-3432-9923-3422
7.Sunday. Olakunle Olarewaju Department of community medicine, Faculty of clinical sciences, Osun State University Osogbo, Nigeria. Email: Sunday.olarewaju@uniosun.edu.eg ORCID: https:// orcid.org/0000-0002=82=9=3410
8.Chukwuemeka.Nnmdi Umetiti Department of Biological Sciences, Faculty of natural sciences, Chukwuemeka Odumegwu Ojukwu University, Uli,
Anambra State, Nigeria. Email: emekau.metiti@coou.ed.ng ORCID: https:// orcid.org/0003-3341-4456-5642
Abstract
Musca domestica (House fly) is one of the most widespread flies of medical importance worldwide and can mechanically transmit pathogenic parasites to humans and animals. Increasing resistance to conventional insecticides and the environmental concerns associated with chemical control have prompted the search for safer alternatives. The study aimed at determining the Phytochemical constituents, anti larvicidal activity, mortality rate and lethal concentration LC50 and LC95 of Citrus aurantifolia bark extracts against M. domestica larvae. The bark samples were collected washed, shade-dried, ground into powder, and extracted by macerating 150g of the powder in 4.5L of distilled water. Housefly larvae were reared under laboratory conditions following standard procedure. The findings show that Phytochemical screening revealed the presence of alkaloids, saponins, flvonoides, and tannins, compounds known for their insecticide properties. Five extract concentration (250, 500, 750, 1000, and 1250ppm) were tested with four replicate each. Larvae mortality was recorded after 24, 48, and 72hours, respectively. The highest mortality (18 larvae, 72%) occurred at 1250ppm after 24 hours, indicating that larvicidal activity increased with concentration. Probit analysis estimated LC50 and LC95 values of 5250ppm and 7875ppm respectively at 72hours, confirming a concentration and time-dependent effect. No larval mortality was observed in the control group. These findings demonstrate that C. aurantifolia bark extract has promising larvicidal activity against M.domestica larvae and may serve as an environmentally friendly alternative to synthetic insecticides for housefly control


























